PA5-14265

antibody from Invitrogen Antibodies

Targeting: TMPRSS2 PRSS10

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-14265

Provider

Invitrogen Antibodies

Product name

TMPRSS2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

1.8 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Expanded ACE2 dependencies of diverse SARS-like coronavirus receptor binding domains.

Roelle SM, Shukla N, Pham AT, Bruchez AM, Matreyek KA
PLoS biology 2022 Jul;20(7):e3001738

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a TMPRSS2 polyclonal antibody (Product # PA5-14265) in HepG2 cell lysates (35 µg per lane).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HepG2 cells using a TMPRSS2 polyclonal antibody (Product # PA5-14265) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

S3 Fig Additional figures of clade 3 RBD pseudovirus infectivities. (A) Immunoblotting of lysate subjected to SDS polyacrylamide gel electrophoresis, collected from unmodified HEK 293T cells, HEK 293T cells transiently transfected with a plasmid encoding TMPRSS2 behind a CMV promoter, 293T landing pad cells stably recombined with a plasmid encoding ACE2 cotranslationally linked to either untagged or cytoplasmically HA-tagged TMPRSS2 using a 2A translational stop-start element. The top images show blots performed with an anti-HA antibody, or 3 different antibodies raised against the human TMPRSS2 protein sequence. The bottom images show beta-actin loading controls. (B) A schematic diagram showing the topological domains of TMPRSS2, its internal autoproteolytic cleavage site, and the peptide sequences used as immunogens during antibody creation. (C) Comparison of ACE2-dependent infectivities observed with HEK 293T cells overexpressing ACE2 only or ACE2 with TMPRSS2. The underlying data can be found in S2 Data , and the source code can be found at https://github.com/MatreyekLab/ACE2_dependence . ACE2, angiotensin converting enzyme-2; RBD, receptor binding domain; SARS-CoV-2, Severe Acute Respiratory Syndrome-related Coronavirus 2. (TIF) Click here for additional data file.