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- Antigen structure
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- Product number
- PA5-14265 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMPRSS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 1.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Expanded ACE2 dependencies of diverse SARS-like coronavirus receptor binding domains.
Roelle SM, Shukla N, Pham AT, Bruchez AM, Matreyek KA
PLoS biology 2022 Jul;20(7):e3001738
PLoS biology 2022 Jul;20(7):e3001738
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a TMPRSS2 polyclonal antibody (Product # PA5-14265) in HepG2 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HepG2 cells using a TMPRSS2 polyclonal antibody (Product # PA5-14265) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- S3 Fig Additional figures of clade 3 RBD pseudovirus infectivities. (A) Immunoblotting of lysate subjected to SDS polyacrylamide gel electrophoresis, collected from unmodified HEK 293T cells, HEK 293T cells transiently transfected with a plasmid encoding TMPRSS2 behind a CMV promoter, 293T landing pad cells stably recombined with a plasmid encoding ACE2 cotranslationally linked to either untagged or cytoplasmically HA-tagged TMPRSS2 using a 2A translational stop-start element. The top images show blots performed with an anti-HA antibody, or 3 different antibodies raised against the human TMPRSS2 protein sequence. The bottom images show beta-actin loading controls. (B) A schematic diagram showing the topological domains of TMPRSS2, its internal autoproteolytic cleavage site, and the peptide sequences used as immunogens during antibody creation. (C) Comparison of ACE2-dependent infectivities observed with HEK 293T cells overexpressing ACE2 only or ACE2 with TMPRSS2. The underlying data can be found in S2 Data , and the source code can be found at https://github.com/MatreyekLab/ACE2_dependence . ACE2, angiotensin converting enzyme-2; RBD, receptor binding domain; SARS-CoV-2, Severe Acute Respiratory Syndrome-related Coronavirus 2. (TIF) Click here for additional data file.
