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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PAB12773 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12773, RRID:AB_10553370
- Product name
- POU5F1 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to internal domain of human, mouse and rat POU5F1.
- Host
- Rabbit
- Vial size
- 100 µg
- Storage
- Store at 4°C. For long term storage store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1.
Zhang Z, Liao B, Xu M, Jin Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Oct;21(12):3042-51
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Oct;21(12):3042-51
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The tissue lysate derived from mouse kidney was immunoprobed by POU5F1 polyclonal antibody (Cat # PAB12773) at 1 : 500.An immunoreactive band is observed around ~34 kDa (1).This band is abolished by pre-incubation with immunizing peptide (2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot Blot : 1 ug peptides was blotted onto NC membrane.A : POU2F1B : POU2F2C : POU5F1Followed by immunoblotting at a 1 : 1000 dilution by :1 : POU2F1 polyclonal antibody (Cat # PAB12771) 2 : POU2F2 polyclonal antibody (Cat # PAB12772) 3 : POU5F1 polyclonal antibody (Cat # PAB12773)
- Validation comment
- Dot Blot (Peptide)
