GTX101497

Emhemmed F, Ali Azouaou S, Zhao Q, Appert-Collin A, Bennasroune A, Schini-Kerth VB, Muller CD, Désaubry L, Fuhrmann G
Cell biology and toxicology 2017 Jun;33(3):295-306

Antroquinonol, a Ubiquinone Derivative from the Mushroom Antrodia camphorata, Inhibits Colon Cancer Stem Cell-like Properties: Insights into the Molecular Mechanism and Inhibitory Targets.

Lin HC, Lin MH, Liao JH, Wu TH, Lee TH, Mi FL, Wu CH, Chen KC, Cheng CH, Lin CW
Journal of agricultural and food chemistry 2017 Jan 11;65(1):51-59

PAK1 confers chemoresistance and poor outcome in non-small cell lung cancer via β-catenin-mediated stemness.

Chen MJ, Wu DW, Wang YC, Chen CY, Lee H
Scientific reports 2016 Oct 7;6:34933

Increased chemoresistance via Snail-Raf kinase inhibitor protein signaling in colorectal cancer in response to a nicotine derivative.

Lee TY, Liu CL, Chang YC, Nieh S, Lin YS, Jao SW, Chen SF, Liu TY
Oncotarget 2016 Apr 26;7(17):23512-20

Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells.

Ju SY, Huang CY, Huang WC, Su Y
Cell death & disease 2015 Jul 2;6(7):e1801

Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel.

Mineda K, Feng J, Ishimine H, Takada H, Doi K, Kuno S, Kinoshita K, Kanayama K, Kato H, Mashiko T, Hashimoto I, Nakanishi H, Kurisaki A, Yoshimura K
Stem cells translational medicine 2015 Dec;4(12):1511-22

Short-term spheroid formation enhances the regenerative capacity of adipose-derived stem cells by promoting stemness, angiogenesis, and chemotaxis.

Cheng NC, Chen SY, Li JR, Young TH
Stem cells translational medicine 2013 Aug;2(8):584-94

miR-196a ameliorates phenotypes of Huntington disease in cell, transgenic mouse, and induced pluripotent stem cell models.

Cheng PH, Li CL, Chang YF, Tsai SJ, Lai YY, Chan AW, Chen CM, Yang SH
American journal of human genetics 2013 Aug 8;93(2):306-12

The influence of spheroid formation of human adipose-derived stem cells on chitosan films on stemness and differentiation capabilities.

Cheng NC, Wang S, Young TH
Biomaterials 2012 Feb;33(6):1748-58

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (20 ug of whole cell lysate) A: human ESC 12% SDS PAGE GTX101497 diluted at 1:10000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (20 ?g of whole cell lysate) A: mouse ESC 12% SDS PAGE GTX101497 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Oct4 antibody detects Oct4 protein by western blot analysis.A. 30 ?g human ESC whole cell extract 12% SDS-PAGEOct4 antibody (GTX101497) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: OCT4 antibody detects 42281 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with OCT4 antibody (GTX101497) at a dilution of 1:50000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:100000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescence analysis of hESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescence analysis of mESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Oct4 protein stained by Oct4 antibody (GTX101497) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 £gg of Oct4 antibody (GTX101497) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX101497) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using OCT3/4(GTX101497) antibody at 1:500 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX101497)antibody at 1:100 dilution(purple) or rabbit IgG (black).