- NB100-2379 - Novus Biologicals POU5F1 antibody

Submitted references Generation of induced pluripotent stem cell line (ZZUi0016-A) from dermal fibroblasts of a normal human.

Gao Y, Wang Y, Wang Z, Sun H, Zhang R, Yang J, Liu Y, Liu H, Zhang Q, Zhang S, Shi C, Xu Y, Song B
Stem cell research 2020 Mar;43:101717

MUC1-C drives stemness in progression of colitis to colorectal cancer.

Li W, Zhang N, Jin C, Long MD, Rajabi H, Yasumizu Y, Fushimi A, Yamashita N, Hagiwara M, Zheng R, Wang J, Kui L, Singh H, Kharbanda S, Hu Q, Liu S, Kufe D
JCI insight 2020 Jun 18;5(12)

Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness.

Lu H, Xie Y, Tran L, Lan J, Yang Y, Murugan NL, Wang R, Wang YJ, Semenza GL
The Journal of clinical investigation 2020 Jul 27;

Generation of induced pluripotent stem cell line (ZZUi0018-A ) from a patient with spinocerebellar ataxia type 6.

Yang T, Qin J, Zhang Q, Sun H, Wang Z, Yang J, Liu H, Zhang C, Zhang S, Zhang J, Wang Y, Xu Y
Stem cell research 2020 Apr;44:101777

The Neonatal and Adult Human Testis Defined at the Single-Cell Level.

Sohni A, Tan K, Song HW, Burow D, de Rooij DG, Laurent L, Hsieh TC, Rabah R, Hammoud SS, Vicini E, Wilkinson MF
Cell reports 2019 Feb 5;26(6):1501-1517.e4

β-Hydroxybutyrate Prevents Vascular Senescence through hnRNP A1-Mediated Upregulation of Oct4.

Han YM, Bedarida T, Ding Y, Somba BK, Lu Q, Wang Q, Song P, Zou MH
Molecular cell 2018 Sep 20;71(6):1064-1078.e5

YM155 as an inhibitor of cancer stemness simultaneously inhibits autophosphorylation of epidermal growth factor receptor and G9a-mediated stemness in lung cancer cells.

Cheng CC, Chang J, Huang SC, Lin HC, Ho AS, Lim KH, Chang CC, Huang L, Chang YC, Chang YF, Wu CW
PloS one 2017;12(8):e0182149

Stem cells and necrotizing enterocolitis: A direct comparison of the efficacy of multiple types of stem cells.

McCulloh CJ, Olson JK, Zhou Y, Wang Y, Besner GE
Journal of pediatric surgery 2017 Jun;52(6):999-1005

Expression of pluripotency markers in the bovine uterus with adenomyosis.

Łupicka M, Socha B, Szczepańska A, Korzekwa A
Reproductive biology and endocrinology : RB&E 2015 Sep 29;13:110

Activation of FGF1B Promoter and FGF1 Are Involved in Cardiogenesis Through the Signaling of PKC, but Not MAPK.

Lin HY, Lee DC, Wang HD, Chi YH, Chiu IM
Stem cells and development 2015 Dec 15;24(24):2853-63

Mitochondrial ribosomal protein S18-2 evokes chromosomal instability and transforms primary rat skin fibroblasts.

Darekar SD, Mushtaq M, Gurrapu S, Kovalevska L, Drummond C, Petruchek M, Tirinato L, Di Fabrizio E, Carbone E, Kashuba E
Oncotarget 2015 Aug 28;6(25):21016-28

Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype.

Lu H, Samanta D, Xiang L, Zhang H, Hu H, Chen I, Bullen JW, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2015 Aug 18;112(33):E4600-9

Leukemia inhibitory factor-induced Stat3 signaling suppresses fibroblast growth factor 1-induced Erk1/2 activation to inhibit the downstream differentiation in mouse embryonic stem cells.

Liu JW, Hsu YC, Kao CY, Su HL, Chiu IM
Stem cells and development 2013 Apr 15;22(8):1190-7

The use of parthenotegenetic and IVF bovine blastocysts as a model for the creation of human embryonic stem cells under defined conditions.

Ruggeri RR, Watanabe Y, Meirelles F, Bressan FF, Frantz N, Bos-Mikich A
Journal of assisted reproduction and genetics 2012 Oct;29(10):1039-43

Stem cell gene expression in MRPS18-2-immortalized rat embryonic fibroblasts.

Yenamandra SP, Darekar SD, Kashuba V, Matskova L, Klein G, Kashuba E
Cell death & disease 2012 Jan 19;3:e357

Long-term recovery of irradiated prostate cancer increases cancer stem cells.

Cho YM, Kim YS, Kang MJ, Farrar WL, Hurt EM
The Prostate 2012 Dec 1;72(16):1746-56

No comments: Submit comment

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: OCT4 Antibody [NB100-2379] - WB detection of OCT4 in mouse brain lysate using OCT4 antibody with ECL based detection. The antibody generated a specific band of OCT4 at the expected ~40kDa position.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: OCT4 Antibody [NB100-2379] - The expression of pluripotency factors Oct4 in human head and neck cancer cell lines.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: OCT4 Antibody [NB100-2379] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and Western blot assay was performed. WB image submitted by a verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: OCT4 Antibody [NB100-2379] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: OCT4 Antibody [NB100-2379] - An intracellular stain was performed on Jurkat with NB100-2379 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

NB100-2379

Antibody data