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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB3868 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3868, RRID:AB_1578961
- Product name
- POU5F1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of POU5F1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors.
Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers.
Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4.
Looijenga LH, Stoop H, de Leeuw HP, de Gouveia Brazao CA, Gillis AJ, van Roozendaal KE, van Zoelen EJ, Weber RF, Wolffenbuttel KP, van Dekken H, Honecker F, Bokemeyer C, Perlman EJ, Schneider DT, Kononen J, Sauter G, Oosterhuis JW
Cancer research 2003 May 1;63(9):2244-50
Cancer research 2003 May 1;63(9):2244-50
Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers.
Reményi A, Lins K, Nissen LJ, Reinbold R, Schöler HR, Wilmanns M
Genes & development 2003 Aug 15;17(16):2048-59
Genes & development 2003 Aug 15;17(16):2048-59
Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4.
Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, Schöler H, Smith A
Cell 1998 Oct 30;95(3):379-91
Cell 1998 Oct 30;95(3):379-91
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The POU5F1 polyclonal antibody (Cat # PAB3868) is used in Western blot to detect POU5F1 in A-375 cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of POU5F1 polyclonal antibody (Cat # PAB3868) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
- Validation comment
- Immunofluorescence
