GTX627419

Gupta N, Badeaux M, Liu Y, Naxerova K, Sgroi D, Munn LL, Jain RK, Garkavtsev I
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 31;114(5):1033-1038

The reciprocal regulation loop of Notch2 pathway and miR-23b in controlling gastric carcinogenesis.

Huang TT, Ping YH, Wang AM, Ke CC, Fang WL, Huang KH, Lee HC, Chi CW, Yeh TS
Oncotarget 2015 Jul 20;6(20):18012-26

Systematic identification of barriers to human iPSC generation.

Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust EM, McManus MT, Song JS, Ramalho-Santos M
Cell 2014 Jul 17;158(2):449-461

Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis.

Wang AM, Huang TT, Hsu KW, Huang KH, Fang WL, Yang MH, Lo SS, Chi CW, Lin JJ, Yeh TS
Oncotarget 2014 Jul 15;5(13):5002-16

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Supportive validation

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Main image
Experimental details: Sample (20 ?g of whole cell lysate) A: mouse ESC 10% SDS PAGE GTX627419 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: NT2D1 10% SDS PAGE GTX627419 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

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Main image
Experimental details: Sample (10 ?g of whole cell lysate) A: Non-transfected HeLa lysates B: HeLa transfected recombinant Oct4 protein 10% SDS PAGE GTX627419 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Oct4 antibody [GT486] detects Oct4 protein by western blot analysis.A. 30 ?g human ESC whole cell lysate/extract10% SDS-PAGE (GTX627419) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Oct4 antibody detects Oct4 protein by immunofluorescent analysis. Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody (GTX627419) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:500.Red: phalloidin, a cytoskeleton? marker, diluted at 1:100.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 £gg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 £gg of Oct4 antibody (GTX627419) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627419) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using OCT3/4(GTX627419) antibody at 1:200 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Oct4 antibody [GT486] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT486] dilution: 1:50