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- Antigen structure
- References [18]
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- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB1759 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Oct-3/4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q01860- Isotype
- IgG
- Antibody clone number
- 240408
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing.
Generation of a GDE heterozygous mutation human embryonic stem cell line WAe001-A-14 by CRISPR/Cas9 editing.
Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer.
Spatial development of gingival fibroblasts and dental pulp cells: Effect of extracellular matrix.
Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9.
HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia.
Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells.
Suppression of OCT4B enhances sensitivity of lung adenocarcinoma A549 cells to cisplatin via increased apoptosis.
The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells.
Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells.
Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins.
Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells.
Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays.
Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells.
Octamer 4 small interfering RNA results in cancer stem cell-like cell apoptosis.
Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
Wu F, Gao G, Pan T, Yang Z, Xu G, Abbas N, Liu Y, Chen Y, Tan S, You K, Ke X, Zhuang Y, Lin X, Yang F, Li YX
Stem cell research 2018 Mar;27:5-9
Stem cell research 2018 Mar;27:5-9
Generation of a GDE heterozygous mutation human embryonic stem cell line WAe001-A-14 by CRISPR/Cas9 editing.
Xu G, Guo D, Wu F, Abbas N, Lai K, Yuan F, You K, Liu Y, Zhuang Y, Wu Y, Xu Y, Chen Y, Yang F, Pan T, Li YX
Stem cell research 2018 Mar;27:38-41
Stem cell research 2018 Mar;27:38-41
Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer.
Wang P, Wan WW, Xiong SL, Feng H, Wu N
Cell death discovery 2017;3:16105
Cell death discovery 2017;3:16105
Spatial development of gingival fibroblasts and dental pulp cells: Effect of extracellular matrix.
Kaufman G, Skrtic D
Tissue & cell 2017 Jun;49(3):401-409
Tissue & cell 2017 Jun;49(3):401-409
Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9.
Xu Y, Wu Y, Guo D, Gao G, Lai K, Yang F, Wang K, Wu H, Lai L, Li J, Xu K, Li YX
Stem cell research 2017 Jul;22:29-32
Stem cell research 2017 Jul;22:29-32
HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia.
Wang P, Lan C, Xiong S, Zhao X, Shan Y, Hu R, Wan W, Yu S, Liao B, Li G, Wang J, Zou D, Chen B, Feng H, Wu N
Oncotarget 2017 Apr 25;8(17):28074-28092
Oncotarget 2017 Apr 25;8(17):28074-28092
Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells.
Hagiwara K, Obayashi T, Sakayori N, Yamanishi E, Hayashi R, Osumi N, Nakazawa T, Nishida K
PloS one 2014;9(1):e84072
PloS one 2014;9(1):e84072
Suppression of OCT4B enhances sensitivity of lung adenocarcinoma A549 cells to cisplatin via increased apoptosis.
Cortes-Dericks L, Yazd EF, Mowla SJ, Schmid RA, Karoubi G
Anticancer research 2013 Dec;33(12):5365-73
Anticancer research 2013 Dec;33(12):5365-73
The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells.
Pekkanen-Mattila M, Ojala M, Kerkelä E, Rajala K, Skottman H, Aalto-Setälä K
Stem cells international 2012;2012:875059
Stem cells international 2012;2012:875059
Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells.
Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS
Molecular therapy : the journal of the American Society of Gene Therapy 2011 Mar;19(3):584-93
Molecular therapy : the journal of the American Society of Gene Therapy 2011 Mar;19(3):584-93
Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins.
Angel M, Yanik MF
PloS one 2010 Jul 23;5(7):e11756
PloS one 2010 Jul 23;5(7):e11756
Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
Semenov OV, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch AH, Malek A
American journal of obstetrics and gynecology 2010 Feb;202(2):193.e1-193.e13
American journal of obstetrics and gynecology 2010 Feb;202(2):193.e1-193.e13
A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
Klim JR, Li L, Wrighton PJ, Piekarczyk MS, Kiessling LL
Nature methods 2010 Dec;7(12):989-94
Nature methods 2010 Dec;7(12):989-94
Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells.
Ware CB, Wang L, Mecham BH, Shen L, Nelson AM, Bar M, Lamba DA, Dauphin DS, Buckingham B, Askari B, Lim R, Tewari M, Gartler SM, Issa JP, Pavlidis P, Duan Z, Blau CA
Cell stem cell 2009 Apr 3;4(4):359-69
Cell stem cell 2009 Apr 3;4(4):359-69
Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays.
Cordey M, Limacher M, Kobel S, Taylor V, Lutolf MP
Stem cells (Dayton, Ohio) 2008 Oct;26(10):2586-94
Stem cells (Dayton, Ohio) 2008 Oct;26(10):2586-94
Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells.
Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ
Stem cells (Dayton, Ohio) 2008 Jun;26(6):1436-43
Stem cells (Dayton, Ohio) 2008 Jun;26(6):1436-43
Octamer 4 small interfering RNA results in cancer stem cell-like cell apoptosis.
Hu T, Liu S, Breiter DR, Wang F, Tang Y, Sun S
Cancer research 2008 Aug 15;68(16):6533-40
Cancer research 2008 Aug 15;68(16):6533-40
Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
Stem cells (Dayton, Ohio) 2007 Feb;25(2):490-9
Stem cells (Dayton, Ohio) 2007 Feb;25(2):490-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Oct-3/4 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Oct-3/4 Monoclonal Antibody (Catalog # MAB1759) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Oct-3/4 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Oct-3/4 in BG01V Human Embryonic Stem Cells. Oct-3/4 was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human/Mouse Oct-3/4 Monoclonal Antibody (Catalog # MAB1759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using Rhodamine Red-coupled anti-rat IgG (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
