Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF4650 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal ISLR-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse ISLR-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human ISLR-2 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Essential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure.
Abudureyimu S, Asai N, Enomoto A, Weng L, Kobayashi H, Wang X, Chen C, Mii S, Takahashi M
Scientific reports 2018 May 8;8(1):7292
Scientific reports 2018 May 8;8(1):7292
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse ISLR-2 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse ISLR-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4650) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ISLR-2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ISLR-2 in Embryonic Mouse Brainstem. ISLR-2 was detected in immersion fixed frozen sections of embryonic mouse brainstem (13 d.p.c.) using Sheep Anti-Mouse ISLR-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4650) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.