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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Immunoprecipitation [2]
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- Product number
- NB100-469 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-469, RRID:AB_2192776
- Product name
- Rabbit Polyclonal SMC5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Inducible Degradation of the Human SMC5/6 Complex Reveals an Essential Role Only during Interphase.
SMC5/6 is required for the formation of segregation-competent bivalent chromosomes during meiosis I in mouse oocytes.
Specialized compartments of cardiac nuclei exhibit distinct proteomic anatomy.
Probing PML body function in ALT cells reveals spatiotemporal requirements for telomere recombination.
Venegas AB, Natsume T, Kanemaki M, Hickson ID
Cell reports 2020 Apr 21;31(3):107533
Cell reports 2020 Apr 21;31(3):107533
SMC5/6 is required for the formation of segregation-competent bivalent chromosomes during meiosis I in mouse oocytes.
Hwang G, Sun F, O'Brien M, Eppig JJ, Handel MA, Jordan PW
Development (Cambridge, England) 2017 May 1;144(9):1648-1660
Development (Cambridge, England) 2017 May 1;144(9):1648-1660
Specialized compartments of cardiac nuclei exhibit distinct proteomic anatomy.
Franklin S, Zhang MJ, Chen H, Paulsson AK, Mitchell-Jordan SA, Li Y, Ping P, Vondriska TM
Molecular & cellular proteomics : MCP 2011 Jan;10(1):M110.000703
Molecular & cellular proteomics : MCP 2011 Jan;10(1):M110.000703
Probing PML body function in ALT cells reveals spatiotemporal requirements for telomere recombination.
Draskovic I, Arnoult N, Steiner V, Bacchetti S, Lomonte P, Londoño-Vallejo A
Proceedings of the National Academy of Sciences of the United States of America 2009 Sep 15;106(37):15726-31
Proceedings of the National Academy of Sciences of the United States of America 2009 Sep 15;106(37):15726-31
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC5 Antibody [NB100-469] - Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, TCMK-1 and NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SMC5 antibody (lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC5 Antibody [NB100-469] - Detection of human and mouse SMC5 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, RKO, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC5 antibody NB100-469 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SMC5 Antibody [NB100-469] - 293T whole cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SMC5 Antibody [NB100-469] - Detection of human SMC5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC5 antibody NB100-469 (lot 3) used for IP at 6 ug per reaction. SMC5 was also immunoprecipitated by a previous lot of this antibody (lot 2). For blotting immunoprecipitated SMC5, NB100-469 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
