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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00010252-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010252-M01, RRID:AB_548360
- Product name
- Mouse Monoclonal SPRY1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SPRY1 - sprouty homolog 1, antagonist of FGF signaling (Drosophila)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Targeted methylation sequencing reveals dysregulated Wnt signaling in Parkinson disease.
Human Sprouty1 suppresses growth, migration, and invasion in human breast cancer cells.
Initial report on differential expression of sprouty proteins 1 and 2 in human epithelial ovarian cancer cell lines.
Zhang L, Deng J, Pan Q, Zhan Y, Fan JB, Zhang K, Zhang Z
Journal of genetics and genomics = Yi chuan xue bao 2016 Oct 20;43(10):587-592
Journal of genetics and genomics = Yi chuan xue bao 2016 Oct 20;43(10):587-592
Human Sprouty1 suppresses growth, migration, and invasion in human breast cancer cells.
Mekkawy AH, Pourgholami MH, Morris DL
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 May;35(5):5037-48
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 May;35(5):5037-48
Initial report on differential expression of sprouty proteins 1 and 2 in human epithelial ovarian cancer cell lines.
Moghaddam SM, Amini A, Wei AQ, Pourgholami MH, Morris DL
Journal of oncology 2012;2012:373826
Journal of oncology 2012;2012:373826
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPRY1 Antibody (3H4) [H00010252-M01] - Analysis of SPRY1 expression in transfected 293T cell line by SPRY1 monoclonal antibody (M01), clone 3H4.Lane 1: SPRY1 transfected lysate(35.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPRY1 Antibody (3H4) [H00010252-M01] - SPRY1 monoclonal antibody (M01), clone 3H4 Analysis of SPRY1 expression in HepG2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: SPRY1 Antibody (3H4) [H00010252-M01] - Detection limit for recombinant GST tagged SPRY1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SPRY1 Antibody (3H4) [H00010252-M01] - Analysis of monoclonal antibody to SPRY1 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.
