Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
Reference
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- Product number
- NBP1-51918 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-51918, RRID:AB_11054507
- Product name
- Goat Polyclonal SPRY1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NP_005832.1 and NP_955359.1 represent varients of the same protein.
- Reactivity
- Human, Mouse
- Host
- Goat
- Antigen sequence
CPSRGQGKPS
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references MicroRNA-21 maintains hematopoietic stem cell homeostasis through sustaining the NF-κB signaling pathway in mice.
Shp2, an SH2-containing protein-tyrosine phosphatase, positively regulates receptor tyrosine kinase signaling by dephosphorylating and inactivating the inhibitor Sprouty.
Hu M, Lu Y, Zeng H, Zhang Z, Chen S, Qi Y, Xu Y, Chen F, Tang Y, Chen M, Du C, Shen M, Wang F, Su Y, Wang S, Wang J
Haematologica 2020 Jan 23;
Haematologica 2020 Jan 23;
Shp2, an SH2-containing protein-tyrosine phosphatase, positively regulates receptor tyrosine kinase signaling by dephosphorylating and inactivating the inhibitor Sprouty.
Hanafusa H, Torii S, Yasunaga T, Matsumoto K, Nishida E
The Journal of biological chemistry 2004 May 28;279(22):22992-5
The Journal of biological chemistry 2004 May 28;279(22):22992-5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPRY1 Antibody [NBP1-51918] - (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: SPRY1 Antibody [NBP1-51918] - analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line)