PA5-58784

antibody from Invitrogen Antibodies

Targeting: SPRYD3 FLJ14800

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-58784

Provider

Invitrogen Antibodies

Product name

SPRYD3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LARKDYPKNR HPGWSRGSVA YHADDGKIFH GSGVGDPFGP RCYKGDIMGC GIMFPRDYIL DSEGDSDDSC DTVILSPTAR AVRNVRNVMY LH

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SPRYD3 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a SPRYD3 Polyclonal Antibody (Product # PA5-58784).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SPRYD3 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a SPRYD3 Polyclonal Antibody (Product # PA5-58784).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of SPRYD3 in human cell line U-2 OS shows positivity in cytoplasm, actin filaments & nucleus but excluded from the nucleoli. Samples were probed using a SPRYD3 Polyclonal Antibody (Product # PA5-58784).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SPRYD3 in human lymph node using SPRYD3 Polyclonal Antibody (Product # PA5-58784).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SPRYD3 in human cerebellum using SPRYD3 Polyclonal Antibody (Product # PA5-58784).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SPRYD3 in human cerebral cortex using SPRYD3 Polyclonal Antibody (Product # PA5-58784).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SPRYD3 in human kidney using SPRYD3 Polyclonal Antibody (Product # PA5-58784).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SPRYD3 in human cerebellum, cerebral cortex, kidney and lymph node using SPRYD3 Polyclonal Antibody (Product # PA5-58784) (A) shows similar protein distribution across tissues to an independent SPRYD3 Polyclonal Antibody (B).