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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-35345 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HLA-B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The effects of p53 gene inactivation on mutant proteome expression in a human melanoma cell model.
The RNA-binding Protein MEX3B Mediates Resistance to Cancer Immunotherapy by Downregulating HLA-A Expression.
Faktor J, Grasso G, Zavadil Kokas F, Kurkowiak M, Mayordomo MY, Kote S, Singh A, Ruidong L, O'Neill JR, Muller P, Goodlett D, Vojtesek B, Hupp T
Biochimica et biophysica acta. General subjects 2020 Dec;1864(12):129722
Biochimica et biophysica acta. General subjects 2020 Dec;1864(12):129722
The RNA-binding Protein MEX3B Mediates Resistance to Cancer Immunotherapy by Downregulating HLA-A Expression.
Huang L, Malu S, McKenzie JA, Andrews MC, Talukder AH, Tieu T, Karpinets T, Haymaker C, Forget MA, Williams LJ, Wang Z, Mbofung RM, Wang ZQ, Davis RE, Lo RS, Wargo JA, Davies MA, Bernatchez C, Heffernan T, Amaria RN, Korkut A, Peng W, Roszik J, Lizée G, Woodman SE, Hwu P
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Jul 15;24(14):3366-3376
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Jul 15;24(14):3366-3376
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HLA-B in 293 cell lysate (2 µg/lane) either nontransfected (lane 1) or transiently transfected (lane 2) with the HLA-B gene using a HLA-B polyclonal antibody (Product # PA5-35345).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HLA-B in A375 cell lysate (35 µg/lane) using a HLA-B polyclonal antibody (Product # PA5-35345).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of A-431 (Lane 1), HL-60 (Lane 2), Jurkat (Lane 3), Ramos (Lane 4), Raji (Lane 5), Daudi (Lane 6) and IMR-32 (Lane 7). The blot was probed with Anti-HLA-B Polyclonal Antibody (Product # PA5-35345, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 43 kDa band corresponding to HLA-B was observed in A-431, HL-60, Jurkat, Ramos and Raji when compared to Daudi and IMR-32 which are reported to be negative for HLA-B expression.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HLA-B was performed using 70% confluent log phase HL-60 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HLA-B Rabbit Polyclonal Antibody(Product # PA5-35345) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HLA-B in formalin-fixed, paraffin-embedded human hepatocarcinoma using a HLA-B polyclonal antibody (Product # PA5-35345) followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HLA-B in HepG2 cells (bottom) compared to a negative control (top) using a HLA-B polyclonal antibody (Product # PA5-35345) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.
