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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-72607 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APEX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.45 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references HMCES safeguards replication from oxidative stress and ensures error-free repair.
Srivastava M, Su D, Zhang H, Chen Z, Tang M, Nie L, Chen J
EMBO reports 2020 Jun 4;21(6):e49123
EMBO reports 2020 Jun 4;21(6):e49123
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of APEX2 in MCF-7 whole cell lysate using 20 µg of protein per lane. Samples were first blocked with 5% NFDM/TBST, incubated with APEX2 polyclonal antibody (Product # PA5-72607) using a dilution of 1:1000 followed by a goat anti-rabbit IgG H&L (HRP) secondary antibody with a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of APEX2 in U251 cells using confocal microscopy. Sample was incubated with APEX2 polyclonal antibody (Product # PA5-72607) using a dilution of 1:25. Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) with a dilution of 1:400 was used as a secondary antibody. Actin was counterstained with Dylight Fluor 554 conjugated Phalloidin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of APEX2 in paraffin-embedded human lung carcinoma tissue. Samples were incubated with APEX2 polyclonal antibody (Product # PA5-72607) followed by a peroxidase-conjugate secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of APEX2 in MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). Samples were incubated with APEX2 polyclonal antibody (Product # PA5-72607) followed by a FITC conjugated goat anti-rabbit secondary antibody.
