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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- GTX101908 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101908, RRID:AB_10619521
- Product name
- VGAT antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX101908 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE GTX101908 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) Boiled and unboiled HeLa whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VGAT antibody [N1N2], N-term detects VGAT protein expression by immunofluorescent analysis.Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using VGAT(GTX101908) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.Sample:Paraffin-Embedded adult mouse retina. Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
