PA5-96231

antibody from Invitrogen Antibodies

Targeting: SLC32A1 bA122O1.1, VGAT, VIAAT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96231

Provider

Invitrogen Antibodies

Product name

VGAT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: Mouse spinal cord, Mouse brain; Cellular Location: Cytoplasmic vesicle membrane, Multi-pass membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.79 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Restoring tripartite glutamatergic synapses: A potential therapy for mood and cognitive deficits in Gulf War illness.

Wang X, Xu Z, Zhao F, Lin KJ, Foster JB, Xiao T, Kung N, Askwith CC, Bruno JP, Valentini V, Hodgetts KJ, Lin CG
Neurobiology of stress 2020 Nov;13:100240

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of VGAT in extracts of various cell lines using VGAT Polyclonal Antibody (Product # PA5-96231) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of VGAT in extracts of various cell lines using VGAT Polyclonal Antibody (Product # PA5-96231) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of VGAT was performed in Y79 cells using VGAT Polyclonal Antibody (Product # PA5-96231) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of VGAT in paraffin-embedded mouse brain using VGAT Polyclonal Antibody (Product # PA5-96231) at a dilution of 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 7 GW-exposure causes impaired glutamatergic synapses, astrocyte atrophy, loss of interneurons, and decreased neurogenesis, which can be restored by LDN/OSU-215111. Upon completion of the behavioral assessments in Fig. 5 , a subset of mice was euthanized for the following pathological studies. (A) GFAP immunohistochemical staining to examine astrocytes. Representative images are shown (scale bars: 100 mum). Boxed areas are shown enlarged in the middle column (scale bars: 25 mum). Quantitative analysis indicates that the GFAP + area decreased in GWI/vehicle mice and was partially restored in LDN/OSU-215111 treated mice. Data was generated from the percentage of GFAP staining in 890 mum x 667 mum area and normalized by control. n = 5-6 animals per group; average of >=6 sections per animal. (B) Parvalbumin immunohistochemical staining to examine parvalbumin-expressing interneurons. Representative images are shown (scale bars: 100 mum). Quantitative analysis indicates that the number of PV + interneurons decreased in GWI/vehicle mice and was partially restored in LDN/OSU-215111 treated mice. n = 9-10 animals per group; average of >=6 sections per animal. (C) Doublecortin immunohistochemical staining to examine newborn neurons in the dentate gyrus. Representative images are shown (scale bars: 100 mum). Quantitative analysis indicates that the number of DCX + cells decreased in GWI/vehicle mice and was partially restored in LDN/OSU-215111 treated mice. n = 9-10 animals per grou