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antibody from Invitrogen Antibodies
Targeting: PHF5A
bK223H9.2, INI, MGC1346, Rds3, SAP14b, SF3b14b, SF3B7
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-56004 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHF5A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AKHHPDLIFC RKQAGVAIGR LCEKCDGKCV ICDSYVRPCT LVRICDECNY GSYQGRCVIC GGPGVSDAYY CKECTIQEKD RDGCPKIVNL GSSKTDLFYE RK Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHF5A in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a PHF5A Polyclonal Antibody (Product # PA5-56004). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHF5A in control (vector only transfected HEK293T lysate) and PHF5A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a PHF5A Polyclonal Antibody (Product # PA5-56004).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PHF5A in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a PHF5A Polyclonal Antibody (Product # PA5-56004).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PHF5A in human duodenum tissue shows nuclear positivity in glandular cells. Samples were probed using a PHF5A Polyclonal Antibody (Product # PA5-56004).
