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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [1]
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- Product number
- PA1-31555 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLK4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLK4 in human Colon lysate (35 µg protein in RIPA buffer) using a PLK4 polyclonal antibody (Product # PA1-31555) at a dilution of 2 µg/mL following incubation for 1 hour and detected using chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PLK4 was performed by loading 35 µg (in RIPA buffer) of human colon lysates. Proteins were transferred to a membrane and probed with a PLK4 Polyclonal Antibody (Product # PA1-31555) at a dilution of 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PLK4 in PFA fixed A431 cells. Cells were permeabilized with 0.15% Triton and PLK4 Polyclonal Antibody (Product # PA1-31555) (Green) at a dilution of 10 µg/mL was used as a primary antibody. Blue: DAPI. Negative control : Unimmunized goat IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PLK4 in PFA fixed HeLa cells. Cells were permeabilized with 0.15% Triton and PLK4 Polyclonal Antibody (Product # PA1-31555) (Green) at a dilution of 10 µg/mL was used as a primary antibody. Blue: DAPI. Negative control : Unimmunized goat IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of TLR3 was performed in HeLa cells (fixed with PFA, permeabilized with 0.5% Triton) using a TLR3 Monoclonal Antibody (40C1285) (Product # MA1-25649) (Blue) at a dilution of 10 µg/mL. Black : Isotype control.
