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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX111754 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111754, RRID:AB_1951290
- Product name
- PLK4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE GTX111754 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLK4 antibody (GTX111754) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PLK4(GTX111754) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PLK4 antibody detects PLK4 protein at cytoplasm and nucleus in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. PLK4 antibody (GTX111754) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PLK4 antibody detects PLK4 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. PLK4 antibody (GTX111754) diluted at 1:500.
