MA5-11642

antibody from Invitrogen Antibodies

Targeting: GP1BA CD42b, GP1B, GPIbalpha

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-11642

Provider

Invitrogen Antibodies

Product name

CD42b Monoclonal Antibody (42C01)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-11642 targets CD42b in IHC (P) and FACS applications and shows reactivity with Human samples.

Antibody clone number

42C01

Concentration

0.2 mg/mL

References

Submitted references

Clot composition characterization using diffuse reflectance spectroscopy in acute ischemic stroke.

Skyrman S, Burström G, Aspegren O, Babic D, Lucassen G, Edström E, Arnberg F, Ohlsson M, Mueller M, Elmi-Terander A, Andersson T
Biomedical optics express 2022 Jun 1;13(6):3311-3323

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High Mobility Group Box 1 Protein in Cerebral Thromboemboli.

Essig F, Babilon L, Vollmuth C, Kollikowski AM, Pham M, Solymosi L, Haeusler KG, Kraft P, Stoll G, Schuhmann MK
International journal of molecular sciences 2021 Oct 19;22(20)

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Structural analysis of ischemic stroke thrombi: histological indications for therapy resistance.

Staessens S, Denorme F, Francois O, Desender L, Dewaele T, Vanacker P, Deckmyn H, Vanhoorelbeke K, Andersson T, De Meyer SF
Haematologica 2020;105(2):498-507

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Redox control of β2-glycoprotein I-von Willebrand factor interaction by thioredoxin-1.

Passam FH, Rahgozar S, Qi M, Raftery MJ, Wong JW, Tanaka K, Ioannou Y, Zhang JY, Gemmell R, Qi JC, Giannakopoulos B, Hughes WE, Hogg PJ, Krilis SA
Journal of thrombosis and haemostasis : JTH 2010 Aug;8(8):1754-62

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Crystal structure of a platelet-agglutinating factor isolated from the venom of Taiwan habu (Trimeresurus mucrosquamatus).

Huang KF, Ko TP, Hung CC, Chu J, Wang AH, Chiou SH
The Biochemical journal 2004 Mar 1;378(Pt 2):399-407

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human tonsil stained with CD42b antibody using peroxidase-conjugate and AEC chromogen. Note staining of platelets in the blood vessels.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD42b in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-11642) at a dilution of 2 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4ºC in the dark. FACS analysis was performed using 400 µL of cell buffer.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 HMGB1 and platelets in cerebral thromboemboli. ( a ) Representative HMGB1 stained thromboemboli and magnifications illustrating the presence of ( b ) small, spotted HMGB1 regions, whereas, especially in peripheral regions, ( c ) extensive areas of HMGB1 were found. ( d ) Representative CD42b stained thromboemboli illustrating the diversity of platelet-rich areas within cerebral thromboemboli. HMGB1 = high mobility group box 1. Scale bars: ( a , d ) 200 um, ( b , c ) 50 um.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Correlation between ( a ) the area of HMGB1 (in % of the total thromboembolus area) and the amount of neutrophils, ( b ) the area of HMGB1 and the area covered by CD42b-positive platelets, and ( c ) the amount of neutrophils with the area covered by CD42b-positive platelets. p = level of significance. r = correlation coefficient. s = Spearman, p = Pearson.