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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunoprecipitation [1]
- Flow cytometry [6]
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- Product number
- NB100-63790 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-63790, RRID:AB_960287
- Product name
- Mouse Monoclonal CD42b/GPIb alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Recognises the human CD42b cell surface antigen, also known as platelet glycoprotein GP1B. CD42b is expressed by platelets and megakaryocytes. Clone AK2 has been reported to block the binding of von Willebrand Factor (VWF) to platelets (1).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Activation-induced changes in platelet surface receptor expression and the contribution of the large-platelet subpopulation to activation.
Moroi M, Farndale RW, Jung SM
Research and practice in thrombosis and haemostasis 2020 Feb;4(2):285-297
Research and practice in thrombosis and haemostasis 2020 Feb;4(2):285-297
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - After labeling the surface proteins of the CHO a beta IX cells with biotin, the GPIbIX complex was immunoprecipitated using the MoAb panel. The presence of the GPIba and GPIX native proteins was shown on the surface of the CHO a beta IX cells. Lane 1 shows the components immunoprecipitated using AK3, lane 2 with AK2, and lane 3 with SZ2. After cleavage with mocarhagin, AK3 precipitated a 70-kD GPIba component and the intact GPIX protein from the surface of the cells (lane 4). AK2 (lane 5) and SZ2 (lane 6) were able to precipitate a 40-kD GPIba component from the supernatant collected after the cleavage step.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets with Mouse anti Human CD42b.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Analysis using the FITC conjugate of NB100-63790. Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD42b:FITC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Analysis using the Biotin conjugate of NB100-63790. Staining of human peripheral blood platelets.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD42b: RPE.
