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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AF4067 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD42b/GPIb alpha Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CD42b/GPIb alpha in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P07359- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The collagen-binding integrin α2β1 is a novel interaction partner of the Trimeresurus flavoviridis venom protein flavocetin-A.
Arlinghaus FT, Eble JA
The Journal of biological chemistry 2013 Jan 11;288(2):947-55
The Journal of biological chemistry 2013 Jan 11;288(2):947-55
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CD42b/GPIb alpha by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD42b/GPIb alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4067) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD42b/GPIb alpha at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CD42b/GPIb alpha in Human Whole Blood CD41+ Platelets by Flow Cytometry. Human whole blood CD41+ platelets were stained with Sheep Anti-Human CD42b/GPIb alpha Polyclonal Antibody (Catalog # AF4067) followed by NL637-conjugated anti sheep antibody (Catalog # NL011) and Human CD41 FITC-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).
