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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- MAB4067 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD42b/GPIb alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CD42b/GPIb alpha in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P07359- Isotype
- IgG
- Antibody clone number
- 486805
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Nanoparticle biointerfacing by platelet membrane cloaking.
Hu CM, Fang RH, Wang KC, Luk BT, Thamphiwatana S, Dehaini D, Nguyen P, Angsantikul P, Wen CH, Kroll AV, Carpenter C, Ramesh M, Qu V, Patel SH, Zhu J, Shi W, Hofman FM, Chen TC, Gao W, Zhang K, Chien S, Zhang L
Nature 2015 Oct 1;526(7571):118-21
Nature 2015 Oct 1;526(7571):118-21
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CD42b/GPIb alpha by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD42b/GPIb alpha Monoclonal Antibody (Catalog # MAB4067) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD42b/GPIb alpha at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD42b/GPIb alpha in Human CD41+ Platelets by Flow Cytometry. Human CD41+ platelets were stained with Mouse Anti-Human Integrin alpha 2b/CD41 PE-conjugated Monoclonal Antibody (Catalog # FAB7616P) and either (A) Mouse Anti-Human CD42b/GPIb alpha Monoclonal Antibody (Catalog # MAB4067) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
