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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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Validation data
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- Product number
- MA1-16595 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Separase Monoclonal Antibody (XJ11-1B12)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This Separase (XJ11-1B12) antibody is useful for Western Blot, where a band is seen at ~220 kDa (full-length form) and ~65 kDa (auto-processed form).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- XJ11-1B12
- Vial size
- 100 µL
- Concentration
- 0.9 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The proteolytic activity of separase in BCR-ABL-positive cells is increased by imatinib.
Haaß W, Stehle M, Nittka S, Giehl M, Schrotz-King P, Fabarius A, Hofmann WK, Seifarth W
PloS one 2012;7(8):e42863
PloS one 2012;7(8):e42863
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Separase detected in 5C6 cell lysates using Product # MA1-16595. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Separase in 5C6 cell lysates. Samples were incubated in Separase monoclonal antibody (Product # MA1-16595). Lane 1: Non-induced lysate; Lane 2: Lysate induced with ponasterone.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Transcript levels, protein levels and proteolytic activity of Separase in BCR-ABL-positive cells treated with IM. Cells were treated individually with distinct concentrations (0.5 to 5 uM) of IM. After about two cell cycle rounds (K562, LAMA-84, 24 h; U937p210BCR-ABL/c6-On, 48 h) total RNA and protein lysates were prepared and analyzed by qRT-PCR (A), Western blot immunostaining (B) and separase fluorometric activity assays (C). For Western blot experiments, Actin served as loading control and/or for densitometric data normalization. Each data point corresponds to one single experiment. Only significant p-values as calculated between treated and untreated cells were shown (see Table 2 for summarized Delta-values). For a representative set of corresponding immunostained Western blots compare Figure 5 panel C. Abbreviations: U937/c6-On, U937 cells expressing a p210BCR-ABL transgene (Tet-On system) after induction with Doxycycline (U937p210BCR-ABL/c6-On).
