PA5-96059

antibody from Invitrogen Antibodies

Targeting: PPP4C PP4, PPX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96059

Provider

Invitrogen Antibodies

Product name

PPP4C Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MAEISDLDRQ IEQLRRCELI KESEVKALCA KAREILVEES NVQRVDSPVT VCGDIHGQFY DLKELFRVGG DVPETNYLFM GDFVDRGFYS VETFLLLLAL KVRYPDRITL IRGNHESRQI TQVYGFYDEC LRKYGSVTVW RYCTEIFDYL SLSAIIDGKI FCVHGGLSPS IQTLDQIRTI DRKQEVPHDG PMCDLLWSDP EDTTGWGVSP RGAGYLFGSD VVAQFNAAND IDMICRAHQL VMEGYKWHFN ETVLTVWSAP NYCYRCGNVA AILELDEHLQ KDFIIFEAAP QETRGIPSKK PVADYFL; Positive Samples: 293T, NIH/3T3, MCF-7, HO-8910PM, Mouse testis, Mouse spleen; Cellular Location: Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.49 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PPP4C in extracts of various cell lines using PPP4C Polyclonal Antibody (Product # PA5-96059) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PPP4C in extracts of various cell lines using PPP4C Polyclonal Antibody (Product # PA5-96059) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PPP4C was performed in MCF-7 cells using PPP4C Polyclonal Antibody (Product # PA5-96059). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PPP4C in paraffin-embedded rat liver using PPP4C Polyclonal Antibody (Product # PA5-96059) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PPP4C in paraffin-embedded human lung cancer using PPP4C Polyclonal Antibody (Product # PA5-96059) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PPP4C in paraffin-embedded human colon using PPP4C Polyclonal Antibody (Product # PA5-96059) at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of PPP4C was performed in 200 µg extracts of 293T cells using PPP4C Polyclonal Antibody (Product # PA5-96059). Western blot was performed from the immunoprecipitate using PPP4C Polyclonal Antibody at a dilution of 1:1000.