Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-109350 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aladin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: GGGVTNLLWS PDGSKILATT PSAVFRVWEA QMWTCERWPT LSGRCQTGCW SPDGSRLLFT VLGEPLIYSL SFPERCGEGK GCVGGAKSAT IVADLSETTI QTPDGEERLG GEAHSMVWDP SGERLAVLMK GKPRVQDGKP VILLFRTRNS PVFELLPCGI IQGEPGAQPQ LITFHPSFNK GALLSVGWST GRIAHIPLYF VNAQFPRFSP VLGRAQEPPA GGGGSIHDLP LFTETSPTSA PWDPLPGPPP VLPHSPHSHL
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.9 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Aladin in extracts of various cell lines. Samples were incubated in Aladin polyclonal antibody (Product # PA5-109350) using a dilution of 1:1,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Aladin in HeLa cells. Samples were incubated in polyclonal Aladin antibody (Product # PA5-109350) using a dilution of 1:50, followed by DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Aladin in paraffin-embedded human appendicitis tissue. Samples were incubated in polyclonal Aladin antibody (Product # PA5-109350) using a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Aladin in paraffin-embedded mouse brain tissue. Samples were incubated in polyclonal Aladin antibody (Product # PA5-109350) using a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Aladin in paraffin-embedded mouse heart tissue. Samples were incubated in polyclonal Aladin antibody (Product # PA5-109350) using a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Aladin in paraffin-embedded mouse lung tissue. Samples were incubated in polyclonal Aladin antibody (Product # PA5-109350) using a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Aladin in paraffin-embedded rat brain tissue. Samples were incubated in polyclonal Aladin antibody (Product # PA5-109350) using a dilution of 1:100.
