LS-C354539

antibody from LSBio

Targeting: ARHGEF9 KIAA0424, PEM-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C354539

Provider

LSBio

Product name

ARHGEF9 / Collybistin Antibody (C-Terminus) LS-C354539

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Rat, Bovine

Host

Rabbit

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of ARHGEF9 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of ARHGEF9 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of ARHGEF9 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemical analysis of ARHGEF9 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w