GTX31229

antibody from GeneTex

Targeting: LAMP1 CD107a

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX31229

Provider

GeneTex

Product name

LAMP1 antibody [4E9/11]

Antibody type

Monoclonal

Reactivity

Porcine

Host

Mouse

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Published customer image: Mouse anti Pig CD107a antibody, clone 4E9/11 used for the detection of CD107a in porcine NK cells by flow cytometry Image caption: NKp46high NK cells in spleen showed the highest cytolytic capacity. The cytolytic capacity of NKp46-defined NK-cell subsets (CD8a+NKp46-: blue, CD8a+NKp46+: green, CD8adim/-NKp46high: red) isolated from spleen was analysed after receptor-mediated degranulation. Cells were stimulated and gated for flow cytometric analysis as outlined in Figure 4. (A) Numbers indicate the percentage of CD107a+ cells and the mean fluorescence intensity of CD107a within respective NKp46-gates. Results are representative of experiments with five different animals. (B) CD107a expression analyses of five animals analysed. The proportion of CD107a+ cells within the different NKp46-defined subsets is shown in the upper graphs. Percentage of CD107a+ NK cells was calculated by subtracting spontaneous degranulation observed in cultures stimulated with isotype-control antibodies from the frequency of CD107a+ cells in cultures stimulated with NKp46 and/or CD16 mAbs. The lower graphs show the mean fluorescence intensity of CD107a within the respective subsets. Mean values are represented by a black bar. Significant differences between the subsets are indicated (* = p From: Mair KH, Mullebner A, Essler SE, Duvigneau JC, Storset AK, Saalmuller A, Gerner W. Porcine CD8adim/-NKp46high NK cells are in a highly activated state. Vet Res. 2013 Mar 1;44:13 .

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Pig peripheral blood granulocytes stained with Mouse anti Pig CD107a followed by Goat anti Mouse IgG:FITC . Membrane permeabilisation was achieved with Leucoperm ™

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Published customer image: Mouse anti Pig CD107a antibody, clone 4E9/11 used for the detection of CD107a in porcine NK cells by flow cytometry Image caption: Analysis of degranulation capacity in NKp46-defined NK-cell subsets in blood. The cytolytic capacity of NKp46-defined NK-cell subsets (CD8a+NKp46-: blue, CD8a+NKp46+: green) isolated from blood was analysed after receptor triggering. Cells were stimulated with rhIL-2 and rpIL-15 overnight. Triggering of NK-receptors was performed by using monoclonal antibodies against NKp46, CD16 or a combination of both. Irrelevant isotype-matched antibody served as negative control. NK-cell receptor mediated degranulation was assessed by measuring the expression of CD107a on the cell surface by four-colour flow cytometry after one hour incubation. CD107a expression was measured on CD3- lymphocytes (not shown), followed by gating on the respective NKp46-defined NK subsets. (A) Numbers indicate the percentage of CD107a+ cells and the mean fluorescence intensity of CD107a within respective NKp46-gates. Results are representative of experiments with five different animals. (B) CD107a expression analyses of five animals analysed. The proportion of CD107a+ cells within the different NKp46-defined subsets is shown in the upper graphs. Percentage of CD107a+ NK cells was calculated by subtracting spontaneous degranulation observed in cultures stimulated with isotype-control antibodies from the frequency of CD107a+ cells in cultures stimulated with NKp46 and/or CD16 mAbs. The lower graphs show the mean fluorescence intensity of CD107a within the respective subsets. Mean values are represented by a black bar. Significant differences between the subsets are indicated (* = p From: Mair KH, Mullebner A, Essler SE, Duvigneau JC, Storset AK, Saalmuller A, Gerner W. Porcine CD8adim/-NKp46high NK cells are in a highly activated state. Vet Res. 2013 Mar 1;44:13 .