328602

antibody from BioLegend

Targeting: LAMP1 CD107a

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

328602

Provider

BioLegend

Proper citation

BioLegend Cat#328602, RRID:AB_1134259

Product name

Purified anti-human CD107a (LAMP-1)

Antibody type

Monoclonal

Antigen

Human adult adherent peripheral blood cells

Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane gly­coprotein. The mature CD107a is heavily glycosylated from a 40 kD core protein. It is expressed by activated platelets, acti­vated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. This molecule is located on the luminal side of lysosomes and has been suggested to play a role in the protection of lysosomes membrane from lysosomal hydrolases. Upon activation, CD107a is transferred to the cell membrane surface. It plays a role in cell adhesion and regula­tion of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin.

Reactivity

Human, Simian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C.

References

Submitted references

Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation.

Watanabe A, Tatematsu M, Saeki K, Shibata S, Shime H, Yoshimura A, Obuse C, Seya T, Matsumoto M
The Journal of biological chemistry 2011 Mar 25;286(12):10702-11

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Hook2 is involved in the morphogenesis of the primary cilium.

Baron Gaillard CL, Pallesi-Pocachard E, Massey-Harroche D, Richard F, Arsanto JP, Chauvin JP, Lecine P, Krämer H, Borg JP, Le Bivic A
Molecular biology of the cell 2011 Dec;22(23):4549-62

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Activated human peripheral blood platelets stained with purified H4A3, followed by anti-mouse IgG FITC

Conjugate

Unconjugated