AM03025PU-N

antibody from Acris Antibodies GmbH

Targeting: LAMP1 CD107a

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

AM03025PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM03025PU-N, RRID:AB_10974381

Product name

anti CD107a / LAMP1

Antibody type

Monoclonal

Antigen

U937 cell line

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

B-T47

Vial size

2ml (200T)

Concentration

0.1 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot analysis using CD107a / LAMP1 Antibody Cat.-No AM03025PU-N (Clone B-T47) at 2 µg/ml in 1 mg/ml lysate, HeLa 10 µg (Lane 1+2), Raw 20µg (Lane 3+4) lysate, blocking buffer (Blotto; 5% dry milk powder in TBST). R1253HRP anti-Mouse IgG (H+L)-HRP as secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

CD107a / LAMP1 Antibody Cat.-No AM03025PU-N (Clone B-T47) on Hela cells at 5 µg/ml (Left), R1404F with F(ab)2 anti Mouse IgG (H+L)-FITC conjugated secondary antibody (1/100). Cells were fixed in 1% PFA, permeabilized in 0.25%Triton X 100 in PBS, blocked in 1% BSA in PBS.