14465-1-AP

antibody from Invitrogen Antibodies

Targeting: SMC6 FLJ22116, SMC6L1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14465-1-AP

Provider

Invitrogen Antibodies

Product name

SMC6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TLEDEAQEN KSKMKMVEEH MEQQKENMEH LKSLKIEAEN KYDAIKFKIN QLSELADPLK DELNLADSEV DNQKRGKRHY EEKQKEHLDT LNKKKRELDM KEKELEEKMS QARQICPERI EVEKSASILD KEINRLRQKI QAEHASHGDR EEIMRQYQEA RETYLDLDSK VRTLKKFIKL LGEIMEHRFK TYQQFRRCLT LRCKLYFDNL LSQRAYCGKM NFDHKNETLS ISVQPGEGNK AAFNDMRALS GGERSFSTVC FILSLWSIAE SPFRCLDEFD VYMDMVNRRI AMDLILKMAD SQRFRQFILL TPQSMSSLPS SKLIRILRMS DPERGQTTLP FRPVTQEEDD DQR (740-1091 aa encoded by B C039828)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.47 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14465-1-AP (SMC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 14465-1-AP (SMC6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 14465-1-AP (SMC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HEK-293 cells using 14465-1-AP (SMC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SMC6 (IP:14465-1-AP, 4ug; Detection:14465-1-AP 1:1000) with mouse testis tissue lysate 4000ug.