Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-470 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-470, RRID:AB_2192787
- Product name
- Rabbit Polyclonal SMC6L1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC6L1 Antibody [NB100-470] - Detection of Human SMC6 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SMC6 antibody NB100-470 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC6L1 Antibody [NB100-470] - Detection of SMC6 in TK6 (lymphoblastoid cells) whole cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SMC6L1 Antibody [NB100-470] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. This antibody is used for IP at 6 ug/mg lysate. SMC6 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated SMC6, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMC6L1 Antibody [NB100-470] - FFPE section of human ovarian tumor. Antibody: Affinity purified rabbit anti-SMC6 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SMC6L1 Antibody [NB100-470] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-SMC6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB