12-5788-42

antibody from Invitrogen Antibodies

Targeting: MICB PERB11.2

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12-5788-42

Provider

Invitrogen Antibodies

Product name

MICA/B Monoclonal Antibody (6D4), PE, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The 6D4 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I chain-related (MIC), MICA and MICB proteins. MICA and MICB are related proteins of 83% amino acid similarity, and show homology with classical human leukocyte antigen (HLA) molecules. The structure of MICA and MICB are similar to classical HLA class I chains, however they do not bind beta2 microglobulin or bind peptide typical of HLA class I. MICA and MICB are expressed on the cell surface of endothelial cells, fibroblasts, gastric epithelium and PHA-stimulated T cells, and act as a ligand for NKGD2 expressed on the surface of NK cells, gammadelta T cells and alpha beta CD8+ T cells. There is evidence to suggest that human cytomegalovirus (HCMV) subverts NK cell detection by inhibiting the function of MICB. Furthermore, MICA and MICB expression has been detected in several epithelial tumours isolated from breast, lung, ovary, prostate, colon and kidney.

Conjugate

Yellow dye

Antibody clone number

6D4

Concentration

5 µL/Test

References

Submitted references

Low dose gemcitabine increases the cytotoxicity of human Vγ9Vδ2 T cells in bladder cancer cells in vitro and in an orthotopic xenograft model.

Shimizu T, Tomogane M, Miyashita M, Ukimura O, Ashihara E
Oncoimmunology 2018;7(5):e1424671

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Role of NKG2D in cytokine-induced killer cells against lung cancer.

Yin X, Lu X, Xiuwen Z, Min Z, Xiao R, Mao Z, Zhang Q
Oncology letters 2017 May;13(5):3139-3143

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Potential for enhanced therapeutic activity of biological cancer therapies with doxycycline combination.

Tang H, Sampath P, Yan X, Thorne SH
Gene therapy 2013 Jul;20(7):770-8

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Plasmodium falciparum-infected erythrocytes induce granzyme B by NK cells through expression of host-Hsp70.

Böttger E, Multhoff G, Kun JF, Esen M
PloS one 2012;7(3):e33774

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of HeLa cells with Mouse IgG2a kappa Isotype Control PE (Product # 12-4724-81) (open histogram) or Anti-Human MICA/B PE (filled histogram). Total viable cells were used for analysis.

Conjugate

Yellow dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were stained with Mouse IgG2a kappa Isotype Control, PE (Product #12-4724-82) (blue histogram) or MICA/B Monoclonal Antibody, PE (purple histogram). Total cells were used for analysis.

Conjugate

Yellow dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Yellow dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4. Changes of MICA/B expression in UBC cells following treatment with gemcitabine and in vitro cytotoxicity assay in MICA/B-knockdown UBC cells. (A) MICA/B expression in T24 and TCCSUP cells before and after ZOL, cisplatin, gemcitabine, and both ZOL and gemcitabine treatment. All agents were used at a concentration of 5 muM for 24 h. MICA/B expression was examined by FACS. A representative histogram is shown. Red histogram: background control; blue: no treatment; orange: agent treatment. (B) Median fluorescence intensity (MFI) of MICA/B expression in each agent-treated sample was quantified and normalized to that of the untreated control sample. Data represent the mean +- SD of triplicate wells. (C) T24 and TCCSUP cells were treated with 5 muM gemcitabine for 24 h. MICA and MICB mRNA transcripts were examined by quantitative RT-PCR. (D) Effects of MICA/B small interfering RNA (siMICA/B). (E) In vitro cytotoxicity assay was performed on MICA/B-knockdown UBC cells. (F) In vitro cytotoxicity assay was performed on UBC cells in the presence of anti-MICA/B blocking mAb or isotype control. (G) In vitro cytotoxicity assay was performed on UBC cells in the presence of anti-NKG2D blocking mAb or isotype control. Data represent the mean +- SD of triplicate wells. Statistical significance is displayed as ** for P < 0.01.

Conjugate

Yellow dye