A301-221A
antibody from Invitrogen Antibodies
Targeting: CHD6
CHD5, dJ620E11.1, FLJ22369, KIAA1335, RIGB
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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Validation data
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- Product number
- A301-221A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHD6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CHD6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, MCF-7, HEK293T, K-562, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD6 antibody (Product # A301-221A; lot A301-221A-2) used for WB at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CHD6 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-CHD6 antibody A301-221A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CHD6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHD6 antibody (Product # A301-221A; lot A301-221A-2) used for IP at 6 µg per reaction. CHD6 was also immunoprecipitated by a previous lot of this antibody (lot A301-221A-1). For blotting immunoprecipitated CHD6, Product # A301-221A was used at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.