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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 17760-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CORO1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSRQVVRSS KFRHVFGQPA KADQCYEDVR VSQTTWDSGF CAVNPKFVAL ICEASGGGAF LVLPLGKTGR VDKNAPTVCG HTAPVLDIAW CPHNDNVIAS GSEDCTVMVW EIPDGGLMLP LREPVVTLEG HTKRVGIVAW HTTAQNVLLS AGCDNVIMVW DVGTGAAMLT LGPEVHPDTI YSVDWSRDGG LICTSCRDKR VRIIEPRKGT VVAEKDRPHE GTRPVRAVFV SEGKILTTGF SRMSERQVAL WDTKHLEEPL SLQELDTSSG VLLPFFDPDT NIVYLCGKGD SSIRYFEITS EAPFLHYLSM FSSKESQRGM GYMPKRGLEV NKCEIARFYK LHERRCEPIA MTVPRKSDLF QEDLYPPTAG PDPALTAEEW LGGRDAGPLL ISLKDGYVPP KSRELRVNRG LDTGRRRAAP EASGTPSSDA VSRLEEEMRK LQATVQELQK RLDRLEETVQ AK (1-461 aa encoded by BC110374)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.22 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HL-60 cells were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat spleen tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 Jurkat cells were intracellularly stained with 0.2 µg Anti-Human CORO1A (Product # 17760-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CORO1A (IP:17760-1-AP, 4ug; Detection:17760-1-AP 1:1000) with mouse spleen tissue lysate 4800ug.
