PA5-96806

antibody from Invitrogen Antibodies

Targeting: SUZ12 CHET9, JJAZ1, KIAA0160

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96806

Provider

Invitrogen Antibodies

Product name

SUZ12 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: CHSRFIFNYV YHPKGARIDV SINECYDGSY AGNPQDIHRQ PGFAFSRNGP VKRTPITHIL VCRPKRTKAS MSEFLESEDG EVEQQRTYSS GHNRLYFHSD TCLPLRPQEM EVDSEDEKDP EWLREKTITQ IEEFSDVNEG EKEVMKLWNL HVMKHGFIAD NQMNHACMLF VENYGQKIIK KNLCRNFMLH LVSMHDFNLI SIMSIDKAVT KLREMQQKLE KGESASPANE EITEEQNGTA NGFSEINSKE KALETDSVSG VSKQSKKQKL; Positive Samples: HeLa, BT-474, MCF-7, 293T; Cellular Location: Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.29 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SUZ12 in extracts from normal (control) and SUZ12 knockout (KO) HeLa cells using SUZ12 Polyclonal Antibody (Product # PA5-96806) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SUZ12 in extracts of various cell lines using SUZ12 Polyclonal Antibody (Product # PA5-96806) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SUZ12 was performed in C6 cells using SUZ12 Polyclonal Antibody (Product # PA5-96806).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SUZ12 in paraffin-embedded rat lung using SUZ12 Polyclonal Antibody (Product # PA5-96806) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SUZ12 in paraffin-embedded rat brain using SUZ12 Polyclonal Antibody (Product # PA5-96806) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SUZ12 in paraffin-embedded rat heart using SUZ12 Polyclonal Antibody (Product # PA5-96806) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SUZ12 in paraffin-embedded human breast cancer using SUZ12 Polyclonal Antibody (Product # PA5-96806) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SUZ12 in paraffin-embedded human gastric cancer using SUZ12 Polyclonal Antibody (Product # PA5-96806) at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of SUZ12 was performed in 200 µg extracts of HeLa cells using SUZ12 Polyclonal Antibody (Product # PA5-96806). Western blot was performed from the immunoprecipitate using SUZ12 Polyclonal Antibody.