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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00145264-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00145264-M01, RRID:AB_426135
- Product name
- SERPINA12 monoclonal antibody (M01), clone 3B3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant SERPINA12.
- Antigen sequence
MNPTLGLAIFLAVLLTVKGLLKPSFSPRNYKALSE
VQGWKQRMAAKELARQNMDLGFKLLKKLAFYNPGR
NIFLSPLSISTAFSMLCLGAQDSTLDEIKQGFNFR
KMPEKDLHEGFHYIIHELTQKTQDLKLSIGNTLFI
DQRLQPQRKFLEDAKNFYSAETILTNFQNLEMAQK
QINDFISQKTHGKINNLIENIDPGTVMLLANYIFF
RARWKHEFDPNVTKEEDFFLEKNSSVKVPMMFRSG
IYQVGYDDKLSCTILEIPYQKNITAIFILPDEGKL
KHLEKGLQVDTFSRWKTLLSRRVVDVSVPRLHMTG
TFDLKKTLSYIGVSKIFEEHGDLTKIAPHRSLKVG
EAVHKAELKMDERGTEGAAGTGAQTLPMETPLVVK
IDKPYLLLIYSEKIPSVLFLGKIVNPIGK- Isotype
- IgG
- Antibody clone number
- 3B3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SERPINA12 expression in transfected 293T cell line by SERPINA12 monoclonal antibody (M01), clone 3B3.Lane 1: SERPINA12 transfected lysate(47.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SERPINA12 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of SERPINA12 transfected lysate using anti-SERPINA12 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SERPINA12 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
