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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-56565 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- YKT6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LCHVYVRNDS LAGVVIADNE YPSRVAFTLL EKVLDEFSKQ VDRIDWPVGS PATIHYPALD GHLSRYQ
- Concentration
- 0.1 mg/mL
Submitted references Tumor Suppressor miR-584-5p Inhibits Migration and Invasion in Smoking Related Non-Small Cell Lung Cancer Cells by Targeting YKT6.
Lee SB, Park YS, Sung JS, Lee JW, Kim B, Kim YH
Cancers 2021 Mar 8;13(5)
Cancers 2021 Mar 8;13(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of YKT6 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a YKT6 Polyclonal Antibody (Product # PA5-56565).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of YKT6 in human pancreas shows strong cytoplasmic positivity in exocrine glandular cells. Samples were probed using a YKT6 Polyclonal Antibody (Product # PA5-56565).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 YKT6 is a direct target gene of miR-584-5p in smoking-related NSCLC cells. ( A ) miR DB (target score >= 80) and Targetscan database were used to analyze the target gene of miR-584-5p. Venn diagrams show groups of predicted target genes. ( B ) Verification of the mRNA expression of candidate target genes by qRT-PCR in H1703 and A549 cells transfected with miR-584-5p mimic or miR control; * p < 0.05, ** p < 0.01, and *** p < 0.001. ( C ) Verification of the protein expression of candidate target genes (HDAC1, YKT6, RAP2A, and ENAH) by Western blot assays in H1703 and A549 cells transfected with the miR-584-5p mimic or miR control; * p < 0.05 and ** p < 0.01. Full Western Blot images can be found in Figure S2 . ( D ) Representative images of the has-miR-584/YKT6 binding sequence. ( E ) Relative luciferase activity was measured in H1703 and A549 cells transfected with the reporter vector containing wild-type or mutant-type YKT6 3'UTR (untranslated region), along with miR-584-5p mimic or miR control; ** p < 0.01. ( F , G ) Expression of YKT6 in xenograft tumor tissue was analyzed by Western blot and immunohistochemical (IHC) staining. Full Western Blot images can be found in Figure S3 .
