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- Antigen structure
- References [16]
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- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- H00151648-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00151648-M01, RRID:AB_548086
- Product name
- Mouse Monoclonal Shugoshin Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SGOL1 - shugoshin-like 1 (S. pombe)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Division of labour between PP2A-B56 isoforms at the centromere and kinetochore.
A positive feedback mechanism ensures proper assembly of the functional inner centromere during mitosis in human cells.
Dataset of Sgo1 expression in cardiac, gastrointestinal, hepatic and neuronal tissue in mouse.
Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation.
Chromosome segregation regulation in human zygotes: altered mitotic histone phosphorylation dynamics underlying centromeric targeting of the chromosomal passenger complex.
Sister kinetochore splitting and precocious disintegration of bivalents could explain the maternal age effect.
Negative feedback at kinetochores underlies a responsive spindle checkpoint signal.
Dynamics of cohesin proteins REC8, STAG3, SMC1 beta and SMC3 are consistent with a role in sister chromatid cohesion during meiosis in human oocytes.
Centromere DNA decatenation depends on cohesin removal and is required for mammalian cell division.
Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation.
A B56gamma mutation in lung cancer disrupts the p53-dependent tumor-suppressor function of protein phosphatase 2A.
Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase.
Phosphorylation of human Sgo1 by NEK2A is essential for chromosome congression in mitosis.
Astrin is required for the maintenance of sister chromatid cohesion and centrosome integrity.
Regulation of mitotic chromosome cohesion by Haspin and Aurora B.
Spred-2 steady-state levels are regulated by phosphorylation and Cbl-mediated ubiquitination.
Vallardi G, Allan LA, Crozier L, Saurin AT
eLife 2019 Mar 4;8
eLife 2019 Mar 4;8
A positive feedback mechanism ensures proper assembly of the functional inner centromere during mitosis in human cells.
Liang C, Zhang Z, Chen Q, Yan H, Zhang M, Xiang X, Yi Q, Pan X, Cheng H, Wang F
The Journal of biological chemistry 2019 Feb 1;294(5):1437-1450
The Journal of biological chemistry 2019 Feb 1;294(5):1437-1450
Dataset of Sgo1 expression in cardiac, gastrointestinal, hepatic and neuronal tissue in mouse.
Song AT, Galli A, Leclerc S, Nattel S, Mandato C, Andelfinger G
Data in brief 2017 Aug;13:731-737
Data in brief 2017 Aug;13:731-737
Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation.
Asghar A, Lajeunesse A, Dulla K, Combes G, Thebault P, Nigg EA, Elowe S
Nature communications 2015 Sep 24;6:8364
Nature communications 2015 Sep 24;6:8364
Chromosome segregation regulation in human zygotes: altered mitotic histone phosphorylation dynamics underlying centromeric targeting of the chromosomal passenger complex.
van de Werken C, Avo Santos M, Laven JS, Eleveld C, Fauser BC, Lens SM, Baart EB
Human reproduction (Oxford, England) 2015 Oct;30(10):2275-91
Human reproduction (Oxford, England) 2015 Oct;30(10):2275-91
Sister kinetochore splitting and precocious disintegration of bivalents could explain the maternal age effect.
Zielinska AP, Holubcova Z, Blayney M, Elder K, Schuh M
eLife 2015 Dec 15;4:e11389
eLife 2015 Dec 15;4:e11389
Negative feedback at kinetochores underlies a responsive spindle checkpoint signal.
Nijenhuis W, Vallardi G, Teixeira A, Kops GJ, Saurin AT
Nature cell biology 2014 Dec;16(12):1257-64
Nature cell biology 2014 Dec;16(12):1257-64
Dynamics of cohesin proteins REC8, STAG3, SMC1 beta and SMC3 are consistent with a role in sister chromatid cohesion during meiosis in human oocytes.
Garcia-Cruz R, Brieño MA, Roig I, Grossmann M, Velilla E, Pujol A, Cabero L, Pessarrodona A, Barbero JL, Garcia Caldés M
Human reproduction (Oxford, England) 2010 Sep;25(9):2316-27
Human reproduction (Oxford, England) 2010 Sep;25(9):2316-27
Centromere DNA decatenation depends on cohesin removal and is required for mammalian cell division.
Wang LH, Mayer B, Stemmann O, Nigg EA
Journal of cell science 2010 Mar 1;123(Pt 5):806-13
Journal of cell science 2010 Mar 1;123(Pt 5):806-13
Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation.
Nozawa RS, Nagao K, Masuda HT, Iwasaki O, Hirota T, Nozaki N, Kimura H, Obuse C
Nature cell biology 2010 Jul;12(7):719-27
Nature cell biology 2010 Jul;12(7):719-27
A B56gamma mutation in lung cancer disrupts the p53-dependent tumor-suppressor function of protein phosphatase 2A.
Shouse GP, Nobumori Y, Liu X
Oncogene 2010 Jul 8;29(27):3933-41
Oncogene 2010 Jul 8;29(27):3933-41
Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase.
Hayashi-Takanaka Y, Yamagata K, Nozaki N, Kimura H
The Journal of cell biology 2009 Dec 14;187(6):781-90
The Journal of cell biology 2009 Dec 14;187(6):781-90
Phosphorylation of human Sgo1 by NEK2A is essential for chromosome congression in mitosis.
Fu G, Ding X, Yuan K, Aikhionbare F, Yao J, Cai X, Jiang K, Yao X
Cell research 2007 Jul;17(7):608-18
Cell research 2007 Jul;17(7):608-18
Astrin is required for the maintenance of sister chromatid cohesion and centrosome integrity.
Thein KH, Kleylein-Sohn J, Nigg EA, Gruneberg U
The Journal of cell biology 2007 Jul 30;178(3):345-54
The Journal of cell biology 2007 Jul 30;178(3):345-54
Regulation of mitotic chromosome cohesion by Haspin and Aurora B.
Dai J, Sullivan BA, Higgins JM
Developmental cell 2006 Nov;11(5):741-50
Developmental cell 2006 Nov;11(5):741-50
Spred-2 steady-state levels are regulated by phosphorylation and Cbl-mediated ubiquitination.
Lock P, I ST, Straffon AF, Schieb H, Hovens CM, Stylli SS
Biochemical and biophysical research communications 2006 Dec 29;351(4):1018-23
Biochemical and biophysical research communications 2006 Dec 29;351(4):1018-23
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Shugoshin Antibody (3C11) [H00151648-M01] - Analysis of SGOL1 expression in transfected 293T cell line by SGOL1 monoclonal antibody (M01), clone 3C11.Lane 1: SGOL1 transfected lysate(33.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Shugoshin Antibody (3C11) [H00151648-M01] - Analysis of SGOL1 transfected lysate using anti-SGOL1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SGOL1 MaxPab rabbit polyclonal antibody.
