Explore
Validate
Learn
Flow cytometry
Radioimmunoassay
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Other assay [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA1-72500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FCAR (soluble) Monoclonal Antibody (MIP7c)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody recognizes the EC1 domain of the FcaRI extracellular region.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MIP7c
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Submitted references C-Reactive Protein Enhances IgG-Mediated Cellular Destruction Through IgG-Fc Receptors in vitro.
Pentraxins and IgA share a binding hot-spot on FcαRI.
FcαRI-mediated inhibition of IL-12 production and priming by IFN-γ of human monocytes and dendritic cells.
Temming AR, Tammes Buirs M, Bentlage AEH, Treffers LW, Feringa H, de Taeye SW, Kuijpers TW, Nagelkerke SQ, Brasser G, Mok JY, van Esch WJE, van den Berg TK, Rispens T, van der Schoot CE, Vidarsson G
Frontiers in immunology 2021;12:594773
Frontiers in immunology 2021;12:594773
Pentraxins and IgA share a binding hot-spot on FcαRI.
Lu J, Marjon KD, Mold C, Marnell L, Du Clos TW, Sun P
Protein science : a publication of the Protein Society 2014 Apr;23(4):378-86
Protein science : a publication of the Protein Society 2014 Apr;23(4):378-86
FcαRI-mediated inhibition of IL-12 production and priming by IFN-γ of human monocytes and dendritic cells.
Lecocq M, Detry B, Guisset A, Pilette C
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 1;190(5):2362-71
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 1;190(5):2362-71
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Radioimmune assay analysis of FCAR in neutrophil phagocytosed IgA immune complexes using an FCAR monoclonal antibody (Product # MA1-72500). Human neutrophils were isolated from blood. IgA immune complexes (IC) were made using anti-NIP IgA2 and 125I-labeled NIP/BSA. 4 x 10^5 neutrophils were incubated with 5 µg IgA IC in the presence or absence of 4 µg of the FCAR monoclonal antibody (Product # MA1-72500, clone # MIP7C) at 37 °C for 3 hours. After the surface-bound IgA IC were solubilized with NIP-CAP-OH, neutrophils containing phagocytosed IgA IC were centrifuged through oil (dinonyl phthalate mixed with dibutyl phthalate). The percentage of IgA IC phagocytosed by neutrophils was calculated according to their radioactivity compared with the radioactivity of the whole IgA IC.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Radioimmune assay analysis of FCAR in neutrophil phagocytosed IgA immune complexes using an FCAR monoclonal antibody (Product # MA1-72500). Human neutrophils were isolated from blood. IgA immune complexes (IC) were made using anti-NIP IgA2 and 125I-labeled NIP/BSA. 4 x 10^5 neutrophils were incubated with 5 µg IgA IC in the presence or absence of 4 µg of the FCAR monoclonal antibody (Product # MA1-72500, clone # MIP7C) at 37 °C for 3 hours. After the surface-bound IgA IC were solubilized with NIP-CAP-OH, neutrophils containing phagocytosed IgA IC were centrifuged through oil (dinonyl phthalate mixed with dibutyl phthalate). The percentage of IgA IC phagocytosed by neutrophils was calculated according to their radioactivity compared with the radioactivity of the whole IgA IC.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 FcgammaRIa is mainly responsible for CRP-mediated enhancement of PMN effector functions. The role of FcRs in IgG1-mediated PMN respiratory burst activity (A) , phagocytosis (B) , and cytotoxicity (C) was determined using a panel of FcR blocking agents in the presence (gray bars) or absence (white bars) of CRP (10 mug/ml). For blocking, effector cells were pre-incubated with the respective blocking agent, or isotype equivalent for control purposes, at a concentration of 10 mug/ml. An IgG1 concentration of 0.125 mug/ml was used for respiratory burst and phagocytosis tests, and 0.01 mug/ml for cytotoxicity assay. For respiratory burst data, gMFI values from conditions without blocking and CRP were normalized to 1.0. Phagocytosis is depicted as phagocytic index (# erythrocytes per 100 neutrophils) and cytotoxicity as the percentage (%) of lysed target erythrocytes. Respiratory burst and cytotoxicity blocking experiments have been performed using G-CSF/IFN-gamma-stimulated PMNs from 4 donors tested in three independent experiments and phagocytosis tests for 5 donors tested in four independent experiments. (D) Similarly, the role of FcRs in IgG2-mediated cytotoxicity was determined in the presence (red bars) or absence (pink bars) of CRP (10 mug/ml), using the same panel of FcR blocking agents and an IgG concentration of 0.5 mug/ml was used. IgG2-based cytotoxicity assays have been performed in three independent experiments using G-CSF/IFN-gamma-stimulated PMNs from 4 Fcga
