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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29831 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SERPINA5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2 conditioned medium.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PAI3 using A) 30 µg 293T whole cell lysate and B) 30 µg H1299 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PAI3 polyclonal antibody (Product # PA5-29831) at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PAI3 in HepG2 whole cell extracts (30 µg) and conditioned medium. Samples were separated by 10% SDS-PAGE and the membrane was probed with PAI3 Polyclonal antibody (Product # PA5-29831) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SERPINA5 Polyclonal Antibody detects PAI3 protein by western blot analysis. HepG2 whole cell extracts (30 µg) and conditioned medium were separated by 10% SDS-PAGE, and the membrane was blotted with SERPINA5 Polyclonal Antibody (Product # PA5-29831) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PAI3 in methanol-fixed HeLa cells using a PAI3 polyclonal antibody (Product # PA5-29831) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SERPINA5 Polyclonal Antibody detects SERPINA5 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. SERPINA5 Polyclonal Antibody (Product # PA5-29831) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
