LS-C133286

antibody from LSBio

Targeting: KRT18

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C133286

Provider

LSBio

Product name

KRT18 / CK18 / Cytokeratin 18 Antibody (clone 2F8) LS-C133286

Antibody type

Monoclonal

Description

Purified from ascites by Protein A

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2F8

Storage

Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

KRT18 monoclonal antibody (M01), clone 2F8 Western blot of KRT18 expression in HeLa.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of KRT18 expression in transfected 293T cell line by KRT18 monoclonal antibody (M01), clone 2F8.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection limit for recombinant GST tagged KRT18 is approximately 0.1 ng/ml as a capture antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged KRT18 is approximately 0.1 ng/ml as a capture antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged KRT18 is approximately 0.1 ng/ml as a capture antibody.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation of KRT18 transfected lysate using anti-KRT18 monoclonal antibody and Protein A Magnetic Bead.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of KRT18 transfected lysate using anti-KRT18 monoclonal antibody and Protein A Magnetic Bead.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of KRT18 transfected lysate using anti-KRT18 monoclonal antibody and Protein A Magnetic Bead.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to KRT18 on formalin-fixed paraffin-embedded human liver tissue. [antibody concentration 3 ug/ml].

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to KRT18 on formalin-fixed paraffin-embedded human liver tissue. [antibody concentration 3 ug/ml].