GTX105624

antibody from GeneTex

Targeting: KRT18

Provider product page for GTX105624

Western blot

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [4]
Comments [0]
Validations
Western blot [3]
Immunocytochemistry [2]
Immunohistochemistry [1]

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Product number: GTX105624 - Provider product page
Provider: GeneTex
Proper citation: GeneTex Cat#GTX105624, RRID:AB_1950645
Product name: Cytokeratin 18 antibody [N2C2], Internal
Antibody type: Polyclonal
Reactivity: Human, Mouse
Host: Rabbit

KRT18 protein structure - GTX105624 shown in red.

Submitted references Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells.

Yu HI, Chou HC, Su YC, Lin LH, Lu CH, Chuang HH, Tsai YT, Liao EC, Wei YS, Yang YT, Lee YR, Chan HL
Journal of pharmaceutical and biomedical analysis 2018 Oct 25;160:344-350

High-efficiency cellular reprogramming with microfluidics.

Luni C, Giulitti S, Serena E, Ferrari L, Zambon A, Gagliano O, Giobbe GG, Michielin F, Knöbel S, Bosio A, Elvassore N
Nature methods 2016 May;13(5):446-52

Resources for the Comprehensive Discovery of Functional RNA Elements.

Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13

Klf5 deletion promotes Pten deletion-initiated luminal-type mouse prostate tumors through multiple oncogenic signaling pathways.

Xing C, Ci X, Sun X, Fu X, Zhang Z, Dong EN, Hao ZZ, Dong JT
Neoplasia (New York, N.Y.) 2014 Nov;16(11):883-99

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (15 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N2C2], Internal (GTX105624) diluted at 1:2000.
KD Reagent Type: siRNA
Downregulation: <50%
KD Reagent Prod no: Proprietary.
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod page: KD Reagent Prod page
Antibody Lot Number: 39890

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample(30 £gg of whole cell lysate)A:H1299B:Hep G2(GTX27900)7.5% SDS PAGEGTX105624 diluted at 1:3000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (15 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N2C2], Internal (GTX105624) diluted at 1:2000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 18 (GTX105624) antibody at 1:200 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Cytokeratin 18 antibody [N2C2], Internal detects Cytokeratin 18 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Cytokeratin 18 protein stained by Cytokeratin 18 antibody [N2C2], Internal (GTX105624) diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human endo mitral ovarian cancer, using KRT18(GTX105624) antibody at 1:100 dilution.