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ImmunocytochemistryAntibody data
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- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX112977 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112977, RRID:AB_10719880
- Product name
- Cytokeratin 18 antibody [N1N3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Huang HJ, Lin CC, Chou HC, Chen YW, Lin ST, Lin YC, Lin DY, Lyu KW, Chan HL
Molecular bioSystems 2014 Dec;10(12):3086-100
Molecular bioSystems 2014 Dec;10(12):3086-100
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (15 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:15000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE GTX112977 diluted at 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:5000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:5000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (15 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:15000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 18(GTX112977) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded hepatoma, using Cytokeratin 18 antibody(GTX112977) antibody at 1:500 dilution.
