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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00003875-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003875-M01, RRID:AB_463842
- Product name
- KRT18 monoclonal antibody (M01), clone 2F8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant KRT18.
- Antigen sequence
MSFTTRSTFSTNYRSLGSVQAPSYGARSVSSAASV
YAGAGGSGSRISVSRSTSFRGGMGSGGLATGIAGG
LAGMGGIQNEKETMQSLNDRPASYLDRVRSLETEN
RRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQI
FANTVDNARIVLQIDNARLAADDFRVKYETELAMR
QSVENDIHGLRKVIDDTNITRLQLETEIEALKEEL
LFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDL
AKIMADIRAQYDELARKNREELDKYWSQQIEESTT
VVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRN
LKASLENSLREVEARYALQMEQLNGILLHLESELA
QTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLE
DGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVS
ETNDTKVLRY- Isotype
- IgG
- Antibody clone number
- 2F8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- KRT18 monoclonal antibody (M01), clone 2F8 Western Blot analysis of KRT18 expression in HeLa ( Cat # L013V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of KRT18 expression in transfected 293T cell line by KRT18 monoclonal antibody (M01), clone 2F8.Lane 1: KRT18 transfected lysate(48.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged KRT18 is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to KRT18 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of KRT18 transfected lysate using anti-KRT18 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with KRT18 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to KRT18 on formalin-fixed paraffin-embedded human liver tissue. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
