Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-29609 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 18 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (86%), Rat (85%), Dog (90%), Bovine (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), A-431 (Lane 3), Caco-2 (Lane 4), Caco-2 treated with IL-6 (100ng/ml for 24hr) (Lane 5) and A-375 (Lane 6). The blot was probed with Anti-Cytokeratin 18 Polyclonal Antibody (Product # PA5-29609, 1:4000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 48 kDa band corresponding to Cytokeratin 18 was observed across the cell lines tested. Also, the protein expression increased in Caco-2 cell line upon IL-6 treatment and was not detected in A-375 which is reported to be negative for Cytokeratin 18.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cytokeratin 18 Polyclonal Antibody (Product # PA5-29609). Sample (30 µg of whole cell lysate). Lane A: Raji. 10% SDS PAGE. Cytokeratin 18 Polyclonal Antibody (Product # PA5-29609) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cytokeratin 18 in paraformaldehyde-fixed HeLa cells using a Cytokeratin 18 polyclonal antibody (Product # PA5-29609) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Cytokeratin 18 was performed in paraffin-embedded mouse urinary bladder tissue using Cytokeratin 18 Polyclonal Antibody (Product # PA5-29609) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Cytokeratin 18 (Product # PA5-29609) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.