ALX-804-598-C100

antibody from Enzo Life Sciences

Targeting: TLR5 FLJ10052, MGC126430, MGC126431, SLEB1, TIL3

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

ALX-804-598-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-598-C100, RRID:AB_2052694

Product name

Toll-like receptor 5 monoclonal antibody (19D759.2)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Protein G-affinity purified.

Reactivity

Human, Mouse, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

19D759.2

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

The myeloid differentiation factor 88 (MyD88) is required for CD4+ T cell effector function in a murine model of inflammatory bowel disease.

Fukata M, Breglio K, Chen A, Vamadevan AS, Goo T, Hsu D, Conduah D, Xu R, Abreu MT
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 1;180(3):1886-94

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TGF-alpha regulates TLR expression and function on epidermal keratinocytes.

Miller LS, Sørensen OE, Liu PT, Jalian HR, Eshtiaghpour D, Behmanesh BE, Chung W, Starner TD, Kim J, Sieling PA, Ganz T, Modlin RL
Journal of immunology (Baltimore, Md. : 1950) 2005 May 15;174(10):6137-43

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Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad.

François S, El Benna J, Dang PM, Pedruzzi E, Gougerot-Pocidalo MA, Elbim C
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3633-42

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Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.

Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis of TLR5 in A) Ramos and B) Raw cell lysate using MAb to Toll-like Receptor 5 (19D759.2) (Prod. No. ALX-804-598) at 2µg/ml.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunohistochemical analysis of TLR5 in formalin-fixed, paraffin-embedded mouse brain tissue using an isotype (top) and to MAb to Toll-like Receptor 5 (19D759.2)Ê(Prod. No. ALX-804-598) (bottom) at 5µg/ml.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Intracellular flow analysis of TLR5 inÊRamos cells usingÊ1ug of MAb to Toll-like Receptor 5 (19D759.2) (Prod. No. ALX-804-598).Ê Shaded histogram represents cells without antibody; green represents isotype control (BD Biosciences);Êorange represents MAb to Toll-like Receptor 5 (19D759.2). Secondary antibody: Goat Anti-Mouse IgG PE.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

ÊÊIntracellular flow analysis of TLR5 inÊhuman PBMC (monocytes)ÊÊusingÊ0.5 ug of MAb to Toll-like Receptor 5 (19D759.2) (Prod. No. ALX-804-598).Ê Shaded histogram represents cells without antibody; green represents isotype control (BD Biosciences);Êred represents MAb to Toll-like Receptor 5 (19D759.2). Secondary antibody: Goat Anti-Mouse IgG PE.