H00079019-M01

antibody from Invitrogen Antibodies

Targeting: CENPM C22orf18, CENP-M, MGC861, Pane1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00079019-M01

Provider

Invitrogen Antibodies

Product name

C22ORF18 Monoclonal Antibody (4C12-2C8)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: MSVLRPLDKL PGLNTATILL VGTEDALLQQ LADSMLKEDC ASELKVHLAK SLPLPSSVNR PRIDLIVFVV NLHSKYSLQN TEESLRHVDA SFFLGKVCFL ATGAGRESHC SIHRHTVVKL AHTYQSPLLY CDLEVEGFRA TMAQRLVRVL QICAGHVPGV SALNLLSLLR SSEGPSLEDL

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4C12-2C8

Vial size

100 µg

Concentration

See Label

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody (M01), clone 4C12-2C8.Lane 1: CENPM transfected lysate (Predicted MW: 19.7 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C22orf18 monoclonal antibody (M01), clone 4C12-2C8 Western Blot analysis of C22orf18 expression in K-562 (Cat # L009V1).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody (M01), clone 4C12-2C8.Lane 1: CENPM transfected lysate (Predicted MW: 19.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged C22orf18 is approximately 0.03 ng/mL as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to MGC861 on HeLa cell. Antibody concentration is 10 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of CENPM transfected lysate using anti-CENPM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CENPM MaxPab rabbit polyclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of CENPM transfected lysate using anti-CENPM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CENPM MaxPab rabbit polyclonal antibody.