12544-1-AP

antibody from Invitrogen Antibodies

Targeting: SQLE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12544-1-AP

Provider

Invitrogen Antibodies

Product name

SQLE Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SRDGRKVTV IERDLKEPDR IVGEFLQPGG YHVLKDLGLG DTVEGLDAQV VNGYMIHDQE SKSEVQIPYP LSENNQVQSG RAFHHGRFIM SLRKAAMAEP NAKFIEGVVL QLLEEDDVVM GVQYKDKETG DIKELHAPLT VVADGLFSKF RKSLVSNKVS VSSHFVGFLM KNAPQFKANH AELILANPSP VLIYQISSSE TRVLVDIRGE MPRNLREYMV EKIYPQIPDH LKEPFLEATD NSHLRSMPAS FLPPSSVKKR GVLLLGDAYN MRHPLTGGGM TVAFKDIKLW RKLLKGIPDL YDDAAIFEAK KSFYWARKTS HSFVVNILAQ ALYELFSATD DSLHQLRKAC FLYFKLGGEC VAGPVGL (143-508 aa encoded by BC017033 )

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.19 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 12544-1-AP ( SQLE Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PC-3 cells using 12544-1-AP (SQLE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 12544-1-AP (SQLE Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 12544-1-AP (SQLE Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12544-1-AP ( SQLE Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12544-1-AP ( SQLE Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SQLE (IP:12544-1-AP, 3ug; Detection:12544-1-AP 1:300) with A549 cells lysate 920ug.