- PA5-41810 - Invitrogen Antibodies SRSF6 antibody

Submitted references Splicing factor SRSF6 mediates pleural fibrosis.

Liang LM, Xiong L, Cheng PP, Chen SJ, Feng X, Zhou YY, Niu Q, Wang M, Chen Q, Song LJ, Yu F, He XL, Xiang F, Wang X, Ye H, Ma WL
JCI insight 2021 May 24;6(10)

The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated Stress Response and Cell Cycle Progression.

Meyer C, Garzia A, Mazzola M, Gerstberger S, Molina H, Tuschl T
Molecular cell 2018 Feb 15;69(4):622-635.e6

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of human Jurkat cell lysate using an anti-SFRS6 polyclonal antibody (Product # PA5-41810).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of SRSF6 was achieved by transfecting T-47D cells with SRSF6 specific siRNAs (Silencer® select Product # s12742, s12741). Western blot analysis (Fig. a) was performed using whole cell extracts from the SRSF6 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with SRSF6 Polyclonal Antibody (Product # PA5-41810, 1 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SRSF6. An uncharacterized band was observed at ~65KDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on modified whole cell extracts (1% SDS) of SH-SY5Y (Lane 1), SK-BR-3 (Lane 2), THP-1 (Lane 3), T-47D (Lane 4), A-431 (Lane 5), A549 (Lane 6), Jurkat (Lane 7) and Karpas 299 (Lane 8). The blot was probed with SRSF6 Polyclonal Antibody (Product # PA5-41810, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 40 kDa band corresponding to SRSF6 was observed across the panel tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of SRSF6 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SRSF6 Polyclonal Antibody (Product # PA5-41810) at 5 µg/mL 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominant nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

PA5-41810

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